A single robust stability-indicating RP-HPLC analytical tool for apigenin quantification in bulk powder and in nanoliposomes: a novel approach

Abstract Background Apigenin (4?, 5, 7-trihydroxyflavone), a flavonoid, is present usually in fruits and vegetables possessing numerous biological properties like antioxidant, anti-viral, antibacterial, anti-inflammatory, chemoprevention activity. So study was aimed to prepare characterize nanoliposomes of apigenin estimate its encapsulation efficiency by stability-assisted reverse-phase (RP)-HPLC method. Results The stability indication the RP-HPLC method developed for apigenin-loaded successfully demonstrated parameters were mainly retention time which 4.21 min, limit detection (LOD) 0.49 ?g/mL, quantification (LOQ) 1.48 %relative standard deviation (RSD) less than 2%. Therefore, HPLC accuracy, linearity, LOD, LOQ, precision, %RSD within range found be satisfactory. Conclusion suitable or estimation with nanoliposomes, this will powerful tool future any pharmaceutical preparations.